Iturin A Extracted From Bacillus subtilis WL-2 Affects Phytophthora infestans via Cell Structure Disruption, Oxidative Stress, and Energy Supply Dysfunction.
Identifieur interne : 000136 ( Main/Exploration ); précédent : 000135; suivant : 000137Iturin A Extracted From Bacillus subtilis WL-2 Affects Phytophthora infestans via Cell Structure Disruption, Oxidative Stress, and Energy Supply Dysfunction.
Auteurs : Youyou Wang [République populaire de Chine] ; Congying Zhang [République populaire de Chine] ; Jiao Liang [République populaire de Chine] ; Lufang Wu [République populaire de Chine] ; Wenbin Gao [République populaire de Chine] ; Jizhi Jiang [République populaire de Chine]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2020.
Abstract
Potato late blight, caused by Phytophthora infestans (Mont.) de Bary, represents a great food security threat worldwide and is difficult to control. Recently, Bacillus spp. have been considered biocontrol agents to control many plant diseases. Here, Bacillus subtilis WL-2 was selected as a potent strain against P. infestans mycelium growth, and its functional metabolite was identified as Iturin A via electrospray ionization mass spectrometry (ESI-MS). Analyses using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that Iturin A caused cell membrane disruption and an irregular internal cell structure. In addition, Iturin A triggered oxidative stress reactions similarly to reactive oxygen species (ROS) in P. infestans cells and caused mitochondrial damage, including mitochondrial membrane potential (MMP), mitochondrial respiratory chain complex activity (MRCCA), and ATP production decline. These results highlight that the cell structure disruption, oxidative stress, and energy supply dysfunction induced by Iturin A play an important role in inhibiting P. infestans. Additionally, B. subtilis WL-2 and Iturin A have great potential for inhibiting P. infestans mycelium growth and controlling potato late blight in the future.
DOI: 10.3389/fmicb.2020.536083
PubMed: 33013776
PubMed Central: PMC7509112
Affiliations:
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Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Congying" sort="Zhang, Congying" uniqKey="Zhang C" first="Congying" last="Zhang">Congying Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Jiao" sort="Liang, Jiao" uniqKey="Liang J" first="Jiao" last="Liang">Jiao Liang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Lufang" sort="Wu, Lufang" uniqKey="Wu L" first="Lufang" last="Wu">Lufang Wu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Wenbin" sort="Gao, Wenbin" uniqKey="Gao W" first="Wenbin" last="Gao">Wenbin Gao</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Jizhi" sort="Jiang, Jizhi" uniqKey="Jiang J" first="Jizhi" last="Jiang">Jizhi Jiang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potato late blight, caused by <i>Phytophthora infestans</i> (Mont.) de Bary, represents a great food security threat worldwide and is difficult to control. Recently, <i>Bacillus</i> spp. have been considered biocontrol agents to control many plant diseases. Here, <i>Bacillus subtilis</i> WL-2 was selected as a potent strain against <i>P. infestans</i> mycelium growth, and its functional metabolite was identified as Iturin A via electrospray ionization mass spectrometry (ESI-MS). Analyses using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that Iturin A caused cell membrane disruption and an irregular internal cell structure. In addition, Iturin A triggered oxidative stress reactions similarly to reactive oxygen species (ROS) in <i>P. infestans</i> cells and caused mitochondrial damage, including mitochondrial membrane potential (MMP), mitochondrial respiratory chain complex activity (MRCCA), and ATP production decline. These results highlight that the cell structure disruption, oxidative stress, and energy supply dysfunction induced by Iturin A play an important role in inhibiting <i>P. infestans</i>. Additionally, <i>B. subtilis</i> WL-2 and Iturin A have great potential for inhibiting <i>P. infestans</i> mycelium growth and controlling potato late blight in the future.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33013776</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>06</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Iturin A Extracted From <i>Bacillus subtilis</i> WL-2 Affects <i>Phytophthora infestans</i> via Cell Structure Disruption, Oxidative Stress, and Energy Supply Dysfunction.</ArticleTitle><Pagination><MedlinePgn>536083</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2020.536083</ELocationID><Abstract><AbstractText>Potato late blight, caused by <i>Phytophthora infestans</i> (Mont.) de Bary, represents a great food security threat worldwide and is difficult to control. Recently, <i>Bacillus</i> spp. have been considered biocontrol agents to control many plant diseases. Here, <i>Bacillus subtilis</i> WL-2 was selected as a potent strain against <i>P. infestans</i> mycelium growth, and its functional metabolite was identified as Iturin A via electrospray ionization mass spectrometry (ESI-MS). Analyses using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that Iturin A caused cell membrane disruption and an irregular internal cell structure. In addition, Iturin A triggered oxidative stress reactions similarly to reactive oxygen species (ROS) in <i>P. infestans</i> cells and caused mitochondrial damage, including mitochondrial membrane potential (MMP), mitochondrial respiratory chain complex activity (MRCCA), and ATP production decline. These results highlight that the cell structure disruption, oxidative stress, and energy supply dysfunction induced by Iturin A play an important role in inhibiting <i>P. infestans</i>. Additionally, <i>B. subtilis</i> WL-2 and Iturin A have great potential for inhibiting <i>P. infestans</i> mycelium growth and controlling potato late blight in the future.</AbstractText><CopyrightInformation>Copyright © 2020 Wang, Zhang, Liang, Wu, Gao and Jiang.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Youyou</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Congying</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.</Affiliation></AffiliationInfo></Author><Author 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